Submitted on December 12, 2007
Revised on June 11, 2008
Accepted on June 12, 2008
The role of desmin in active force transmission and maintenance of structure during growth of urinary bladder
Rolf Sjuve Scott1, Zhenlin Li, Denise Paulin2, Bengt Uvelius1, J. Victor Small3, and Anders Arner4*
1 Lund University
2 Paris VII University
3 Austrian Academy of Sciences
4 Karolinska Institutet
* To whom correspondence should be addressed. E-mail: Anders.Arner{at}ki.se.
The role of the intermediate filament protein desmin in hypertrophy of smooth muscle was examined in desmin deficient mice (Des -/-). A partial obstruction of the urethra was created and after 9-19 days bladder weight increased about 3-fold in both Des -/- and wild type (Des +/+) animals. Bladder growth was associated with synthesis of actin and myosin. In the hypertrophic Des +/+ bladder the relative content of desmin increased. In Des -/- mice desmin was absent. No alterations in the amount of vimentin were observed. Although Des -/- obstructed bladders were capable of growth they had structural changes with partial disruption of the wall. Des-/- bladders had slightly lower passive stress and significantly lower active stress compared to Des+/+. Des-/- preparations had lower shortening velocity. During hypertrophy these structural and mechanical alterations in the Des-/- urinary bladder became more pronounced. In conclusion, desmin in the bladder smooth muscle is not needed for growth but has a role in active force transmission and maintenance of wall structure.
